• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2016 Fiscal Year Final Research Report

Extracellular calcium increases gene expression of fibroblast growth factor-2 via a PKA and ERK1/2 pathway in mouse dental papilla cells.

Research Project

  • PDF
Project/Area Number 26462868
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionTohoku University

Principal Investigator

Kanaya Sousuke  東北大学, 歯学研究科, 助教 (80375097)

Project Period (FY) 2014-04-01 – 2017-03-31
Keywords歯乳頭細胞 / 細胞外Ca2+ / FGF-2
Outline of Final Research Achievements

We have previously reported that elevated extracellular Ca2+ increased bone morphogenetic protein-2 (BMP-2) expression in human dental pulp (hDP) cells. In the present study, we examined the effect of extracellular Ca2+ on FGF-2 gene expression in immortalized mouse dental papilla cells (mDP). Extracellular Ca2+ increased FGF-2 gene expression in mDP. Gene expression of calcium-sensing receptor and G protein-coupled receptor family C group 6 member A, both of which are known to be sensors for extracellular Ca2+, was not detected in mDP. Ca2+-mediated Fgf-2 gene expression was reduced by pretreatment with H-89, a protein kinase A (PKA) inhibitor, or PD98059, an ERK1/2 inhibitor, but not by pretreatment with GF-109203X, a PKC inhibitor, or SB203580, a p38 inhibitor. These findings indicate that elevated extracellular Ca2+ increased Fgf-2 gene expression through PKA and ERK1/2 in mDP cells, and that this mechanism may assist in designing regenerative therapies for dentin.

Free Research Field

歯内治療系歯学

URL: 

Published: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi