2016 Fiscal Year Final Research Report
Reactivation of Epstein-Barr virus by periapical periodontitis-related bacteria
| Project/Area Number |
26462897
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Research Collaborator |
MAKINO Kosuke
KOBAYASHI Hiroshi
IBI Haruna
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 根尖性歯周炎 / 歯根肉芽腫 / ウイルス / 再活性化 / Porphlomonas endodontais / EBER / in situ hybridization / ZEBRA |
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| Outline of Final Research Achievements |
Sixty three periapical lesions were examined, and fifty periapical granulomas were obtained. Thirty eight out of 50 periapical granulomas involved Epstein-Barr virus (EBV). Six out of 9 samples showed EBER mRNA and LMP-1 protein expressing cells. Six out of 9 samples involved Polphylomonas endodontalis. As determined using high performance liquid chromatography, n-butylic acid was most produced in the culture supernatants of P. endodontalis. EBV-infecte cells were cultured with the culture supernatants of P. endodontalis, and the cells expressed ZEBRA protein in the dose dependent manner.
According to these findings, it is concluded that periapcal granulomas were infected with EBV, and EBV was reactivated by n-butyric acid produced from P. endodontalis.
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| Free Research Field |
歯内療法
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