2016 Fiscal Year Final Research Report
Development of biocompatible direct pulp capping cements and the biological effects of the cements on the dental pulp stem cell activities
| Project/Area Number |
26462902
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
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| Research Collaborator |
Hasegawa Tomoya
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 直接覆髄 / リン酸カルシウムセメント / 歯髄由来幹細胞 / 被蓋硬組織誘導 / 生体親和性 / バイオマテリアル / バイオアクティブ |
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| Outline of Final Research Achievements |
To develop a novel α-TCP / Te-CP cement, 2 M sodium dihydrogen phosphate solution was selected for the cement. To examine the biocompatibility of the cement, we prepared the cement-immersed media. The calcium hydroxide (Ca(OH)2) formulation was used as a control. Effects of the eluate from these cement were examined using human dental pulp derived stem cells (hDPSC). Our results revealed that the eluate from our cement did not inhibit cell proliferation, contributing to an increase in ALP activity and in the expression of osteoblast marker genes. In addition, in the animal studies of direct pulp capping, TUNEL positive cells were prominently found in the Ca(OH)2 capped grope, however, in our cement group, there were no significant inflammation findings. In addition, hard tissue regeneration was observed in our cement group. These results suggested that our novel cements indicated both cytocompatible and tissuecompatible properties for pulpal tissue as a direct pulp capping agent.
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| Free Research Field |
歯科保存学
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