2016 Fiscal Year Final Research Report
Functional analysis of autophagy-related genes LC3 and APG5 in dental pulp regeneration using stem cells
| Project/Area Number |
26462903
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | オートファジー / 歯髄再生 |
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| Outline of Final Research Achievements |
In this study, we examined the upregulation of autophagy-related gene (APG) signaling during human odontoblastic differentiation derived from human skeletal muscle stem cells. In a screening experiment, the mRNA and protein levels of microtubule-associated protein 1 light chain (LC3) and APG5, together with the amounts of autophagosomes and autophagy fluxes. Treatment with siRNAs against APG5 suppressed the odontoblast differentiation.
Taken together with our previous findings, we have revealed a unique sequential cascade involving APG5, and MMP-3 in odontoblastic cell differentiation of human skeletal muscle stem cells.
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| Free Research Field |
医歯薬学
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