Development of new dental pulp regeneration method using odontogenesis-mimicking matrix and pluripotent stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462904
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: Ozeki Nobuaki 愛知学院大学, 歯学部, 講師 (70469005)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 脱細胞化

Outline of Final Research Achievements

Pluripotent stem cells can differentiate into multiple cell lineages following exposure to specific microenvironmental signals. We identified unique conditions that mouse pluripotent stem cells（mpSC）to differentiate into odontogenic cell lineages. Isolated acellular mimicking extracellular matrices (mECM) were sufficient to promote cell differentiation along odontoblastic pathways. The mpSC interactions with mECMs and their subsequent differentiation was suppressed by function-blocking integrin antibody. Furthermore, we found that mpSC cultured on the mECM induced the expression and secretion of a set of specific growth factors.
These results show that mpSC odontogenic cell lineage commitment is mediated by integrin interactions with specific ECM microenvironment substrates, leading to unique autocrine growth factor secretion.

Free Research Field

医歯薬学