Protein interactions in periosteum-derived cells determine their three dimensional structure

2016 Fiscal Year Final Research Report

• Project/Area Number: 26462986
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: AKIYAMA Mari 大阪歯科大学, 歯学部, 助教 (60340618)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 骨膜由来細胞 / 骨再生 / タンパク質相互作用 / 質量分析 / ２重免疫染色

Outline of Final Research Achievements

In a previous study, I investigated samples of culture supernatants using a combination of mass spectrometry and single immunohistochemistry. This method identified some proteins expressed in bovine periosteum-derived cells. In this study, I investigated the interactions of these proteins.Double immunohistochemistry was carried out to reveal interactions of type 1 procollagen and other proteins. The results showed that type 1 procollagen overlapped with TMX2. Therefore, type 1 procollagen and TMX2 may interact with each other.
FBXW2 (F-box/WD repeat-containing protein 2) was found in the periosteum, while other proteins were expressed in periosteum-derived cells., Mass spectrometry was carried out using cytoplasmic extracts. Immunohistochemistry of candidate protein ARHGAP36 revealed that this protein was not always expressed in periosteum-derived cells.

Free Research Field

再生医療