Investigation of DNA recognition system in oral mucosa

2016 Fiscal Year Final Research Report

• Project/Area Number: 26463010
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Nishi Hiromi 広島大学, 病院(歯), 助教 (70403558)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 口腔粘膜疾患

Outline of Final Research Achievements

To investigate DNA viral recognition mechanism in oral mucosal cells, we examined the effects of double-strand DNA (dsDNA) derived HSV on anti-viral and inflammatory response in immortalized oral keratinocytes (RT7) and gingival fibroblasts (GT1). We found that CXCL10, a chemokine associated with regulation of T cells, was induced by transfected ds DNA. However, naked dsDNA did not affect the CXCL10 expression. Transfected DNA increased the expression of NF-kB in nuclear fraction, and Ikba　inhibitor decreased CXCL10 expression in both cell in dose dependent manner. CXCL10 expression were inhibited by RIG-I siRNA in both cells, whereas IFI16 siRNA decreased CXCL10 expression in GT1, but not RT7. Oral keratinocytes and fibroblasts recognized dsDNA via intercellular receptor to produce anti-viral chemokines through NF-kB activation, and RIG-I and IFI16 may have a role in regulating these immune responses.

Free Research Field

口腔外科