Epithelial and mesenchymal cross-talk on mechanism of bone invasion of ameloblastoma

2017 Fiscal Year Final Research Report

• Project/Area Number: 26463019
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kagoshima University
• Principal Investigator: NAKAMURA Norifumi 鹿児島大学, 医歯学域歯学系, 教授 (60217875)
• Co-Investigator(Kenkyū-buntansha): 岸田 昭世 鹿児島大学, 医歯学域医学系, 教授 (50274064) ; 岐部 俊郎 鹿児島大学, 医歯学域附属病院, 助教 (50635480) ; 岸田 想子 鹿児島大学, 医歯学域医学系, 助教 (40274089)
• Co-Investigator(Renkei-kenkyūsha): KIBE Toshiro 鹿児島大学, 医歯学域歯学系, 助教 (50635480)
• Research Collaborator: KISHIDA Michiko 鹿児島大学, 医歯学域医学系, 助教 (40274089)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 歯原性腫瘍 / 上皮-間葉相互作用 / エナメル上皮腫 / 骨浸潤

Outline of Final Research Achievements

Analyses of the interaction, cross-talk, between ameloblstoma cell (AM-3) and fibroblast cell (HFF-2) revealed that AM-3 secreted IL-α and facilitated of IL-6 and IL-8 secretion of HFF-2 fibroblasts, and stimulation by the cocultured supernatant of both cells accelerated the migration activity of AM-3. These findings suggest that the ameloblastoma cells and stromal fibroblasts behave interactively via cytokines to create a microenvironment that leads to the extension of ameloblastomas.
Three dimensional observation using double layered collagen gel hemisphere culture revealed that AM-3 cells formed tuft-like invasive processes and collectively invaded into outer layer. These findings were more apparent under the presence of HEF-2 fibroblasts when compared to the single culture of AM-3. HFF-2 fibroblasts localized to the tips of the invasive tumor processes, suggesting that tumor-associated cells assist tumor cell invasion.

Free Research Field

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