Development of gene therapy for oral cancer with direct injection of Naked DNA of alpha2-antiplasmin gene

2016 Fiscal Year Final Research Report

• Project/Area Number: 26463044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Hiroshima University
• Principal Investigator: HAMANA TOMOAKI 広島大学, 医歯薬保健学研究院(歯), 助教 (40397922)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: α2-アンチプラスミン / プラスミン / E-カドヘリン / Naked DNA / 口腔癌遺伝子治療

Outline of Final Research Achievements

SCCKN and alpha2-AP transfectants were subcutaneously inoculated into the nude mice. E-cadherin on cell membrane of xenograft from alpha2-AP transfectants was increased compared to SCCKN. Moreover, beta-catenin increased on cell membrane of xenograft from alpha2-AP transfectants.
These findings suggest that the suppression of the plasminogen activator/plasmin system by alpha2-AP might reduce the dissemination of OSCC cells. Therefore, it could be expected the development of gene therapy for invasiveness and metastasis of OSCC cells with induction of alpha2-AP into OSCC tissue.

Free Research Field

外科系歯学