2016 Fiscal Year Final Research Report
Selection of DSBs repair pathways in heat-induced cell death mediated by BRCA2
| Project/Area Number |
26463052
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 温熱 / 相同組み換え修復 / DNA二本鎖切断 / BRCA2 |
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| Outline of Final Research Achievements |
We showed that heat-induced DSBs are repaired by HR pathways. If phosphorylation of Ku was important for heat-induced DSBs, these phosphorylated enzyme (ATR etc.) inhibitors could block phosphorylation and disassociation from DSBs of Ku. In the colony forming assays, combination use with ATR inhibitor (VE-821) and heat was more sensitive than heat only. In human tongue squamous cell carcinoma cells, immunocytochemical staining for γH2AX following heat showed that the expression in combination use with VE-821 and heat was about twice as much as heat only. This results suggest that phosphorylation of Ku might be important for heat-induced DSBs
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| Free Research Field |
外科系歯学
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