Bone regeneration using condensing platelet fibrin gel and adipose-derived stem cells conditioned medium

2016 Fiscal Year Final Research Report

• Project/Area Number: 26463057
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tohoku University
• Principal Investigator: ITO HIDETOSHI 東北大学, 歯学研究科, 大学院非常勤講師 (80384303)
• Co-Investigator(Renkei-kenkyūsha): FUKUOKA HIROTAROU 東北大学, 大学院歯学研究科, 大学院非常勤講師 (30568746)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 再生 / 骨 / 脂肪幹細胞抽出蛋白

Outline of Final Research Achievements

The objective of this research is to investigate the effect to bone regeneration of condensing platelet fibrin gel derived from autologous blood (Concentrated Growth Factors: CGF) and adipose-derived stem cells conditioned medium(ADSCs-CM). In culture examination, the behavior of angiogenesis of CGF was confirmed. The CGF implanted subcutaneously in rabbit back was disappeared before 4 weeks after implantation. In process of disappearance, many macrophages and newly formed vessels was seen around the CGF. The compressed and membranal CGF showed excellent mechanical properties characterized by high ductility with wide clinical application. The ADSC-MC contained PDGF, VEGF, HGF,and TGF-b, which are known to be necessary for tissue regeneration. This medium contained a high level of VEGF, HGF and TGF-b.

Free Research Field

口腔外科学