2017 Fiscal Year Final Research Report
The role of ubiquitin proteasome system in cardioprotection by volatile anesthetics
Project/Area Number |
26463081
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Osaka Kyoiku University (2015-2017) Osaka Dental University (2014) |
Principal Investigator |
Miyamae Masami 大阪教育大学, 保健センター, 教授 (20298821)
|
Co-Investigator(Kenkyū-buntansha) |
竹村 元三 朝日大学, 歯学部, 教授 (40283311)
金田 一弘 大阪歯科大学, 歯学部, 講師(非常勤) (90533886)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Keywords | sevoflurane / ischemia-reperfusion / heart / ubiquitin-proteasome / guine pig |
Outline of Final Research Achievements |
Administration of sevoflurane prior to ischemia-reperfusion confers cardioprotective effects (sevoflurane preconditioning). We examined the role of ubiquitin proteasome system in this protective effects. Isolated guinea pigs hearts were subjected to 30 min ischemia, followed by 120 min reperfusion (CTL). Anesthetic preconditioning was elicited with 2% sevoflurane for ten minutes prior to ischemia (SEVO). Infarct size was reduced in SEVO compared with CTL. Immunohistochemical analysis revealed that there were less myoglobin-negative cells and ubiquitin and LC 3 stain were increased in SEVO. Electron microscopic study showed that there were more abundant autophagosomes in SEVO. These results suggests that ubiquitin proteasome system plays a role in cardioprotective effects of sevoflurane associated with autophagy.
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Free Research Field |
循環器内科
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