Molecular basis of amelogenin-induced periodontal tissue regeneration

2016 Fiscal Year Final Research Report

• Project/Area Number: 26463137
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Kyushu University
• Principal Investigator: Fukuda Takao 九州大学, 歯学研究院, 助教 (80507781)
• Co-Investigator(Renkei-kenkyūsha): UCHIUMI Takeshi 九州大学, 医学研究院, 准教授 (80253798)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: アメロジェニン / 歯周組織 / 再生 / GRP78 / 歯根膜幹細胞

Outline of Final Research Achievements

Amelogenin, the major component of enamel matrix derivative (Straumann® Emdogain), is well recognized in periodontology. It is used in periodontal surgery to regenerate cementum, periodontal ligament, and alveolar bone. However, the precise molecular mechanisms underlying periodontal regeneration are still unclear.In order to gain further insight into how amelogenin induces periodontal tissue regeneration, we conducted a protein interaction screen using recombinant full-length amelogenin (rM180) as bait. Using this proteomic screen we identified glucose-regulated protein 78 (Grp78) as an amelogenin-binding protein.our study demonstrates that the biological interaction of Grp78 with amelogenin enhances amelogenin-induced cell migration in PDLSCs. Our findings provide a better understanding of the molecular basis of amelogenin-induced periodontal regeneration.

Free Research Field

歯周病学