2017 Fiscal Year Final Research Report
A novel colorimetric system using anthocyanins as a clinical pH indicator
| Project/Area Number |
26463153
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
森田 学 岡山大学, 医歯薬学総合研究科, 教授 (40157904)
竹原 順次 北海道大学, 歯学研究院, 助教 (60216934)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | 安静時唾液 / 唾液ｐＨ / アントシアニン / 簡易測定キット |
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| Outline of Final Research Achievements |
This study was performed to develop a novel colorimetric system using anthocyanins as a pH indicator. Anthocyanins prepared from red wine and red currant extract were characterized by HPLC analysis, alcohol content, and pH. The stability of anthocyanin in aqueous solution and the solid phase was compared. Anthocyanin on a cotton swab was hardly affected by storage for 60 days at room temperature, but the anthocyanin strikingly decreased in aqueous solution.Cotton swabs in which anthocyanin was absorbed displayed a variety of color variations in the pH range from 5.5 to 8.0. Interestingly, it was shown that the color changes correlated with the color component of a* (redness), which was quantified using a chromometer. Measurements of pH in saliva were reproducible for the visual color and color component of a*. These results show that the assay system can be used as a visual indicator for clinical measurement of pH.
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| Free Research Field |
予防歯科学
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