Molecular mechanism of stem cell-inducing reprogramming by epithelial mesenchymal transition

2016 Fiscal Year Final Research Report

• Project/Area Number: 26501006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Regenerative medicine
• Research Institution: Teikyo University of Science & Technology
• Principal Investigator: Masaki Toshihiro 帝京科学大学, 医療科学部, 教授 (00585028)
• Research Collaborator: Anura Rambukkana University of Edinburgh, MRC Centre for Regenerative Medicine, Professor
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: リプログラミング / ハンセン病原因菌 / 上皮間葉転換 / 幹細胞 / 間葉系幹細胞 / シュワン細胞

Outline of Final Research Achievements

Snai1 was introduced into IMS32(immortalized mouse Schwann cell line) or adult mouse primary Schwann cells using lentivirus. In both Snai1-introduced Schwann cells, their morphologies changed to fibroblast-like appearance suggesting that epithelial mesenchymal transition (EMT) occurred. Also IMS32 acquired sphere-forming capacity, and differentiation potential to oil red O-positive preadipocyte-like cells, while differentiation potential to chondrocytes was not seen. On the other hand, Snai1 introduction did not induce these stem cell-like properties in adult mouse primary Schwann cells. These results will be molecular basis for regenerative therapy using these reprogrammed Schwann cell-derived stem cell-like cells.

Free Research Field

幹細胞生物学