2017 Fiscal Year Final Research Report
Development of new biological taxonomy by crystalline crystallin proteome (Crystallome) analysis
| Project/Area Number |
26505002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | University of Fukui |
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Principal Investigator |
Tanaka Yukie 福井大学, 学術研究院医学系部門, 助教 (10197486)
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| Co-Investigator(Kenkyū-buntansha) |
藤井 豊 福井大学, 学術研究院医学系部門, 教授 (80211522)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | クリスタリン / プロテオーム |
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| Outline of Final Research Achievements |
Lens crystalline protein Crystallin has been diverted to existing proteins randomly during their evolution depending on individual species. In order to elucidate the origin protein of bullfrog specific ρ-crystallin, mRNA sequence of ρ-crystallin-like protein present in each tissue was analyzed and its characteristics were analyzed.
As a proteome analysis method to support crystallome, we will evaluate Py-Tag, a novel stable isotope labeling reagent that specifically labels the amino group of lysine residues, and proceed to application to peptides and proteins Basic data was collected. After that, I thought about developing it for application to crystallin analysis.
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| Free Research Field |
生化学
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