2016 Fiscal Year Final Research Report
Development of a platform for proteome analysis of clinical FFPE samples
| Project/Area Number |
26505005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
|
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | プロテオミクス / FFPE / LC-MS |
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| Outline of Final Research Achievements |
This study provided a novel protein-extraction method and highly sensitive LC-MS system suitable for proteome profiling of minuscule FFPE tissue slices. This system can be utilized as a standard platform for FFPE proteome analysis. It was suggested that hematoxylin and eosin staining caused loss of proteins from FFPE tissues. Therefore, more detailed analysis is required for evaluating whether HE-stained FFPE tissue slices are suitable for proteome profiling.
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| Free Research Field |
プロテオミクス
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