2015 Fiscal Year Research-status Report
環境保全微生物に生じたDNAダメージと修復のアダクトミクスに関する研究
| Project/Area Number |
26505010
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
Robert Kanaly 横浜市立大学, 生命ナノシステム科学研究科, 教授 (00448656)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | DNA adductomics |
|---|
| Outline of Annual Research Achievements |
Exposure assays with hydrogen peroxide were conducted using the model organism. DNA adducts were identified in bacterial DNA. Relative levels of some adducts were measured and differences were observed when comparing exposed and unexposed organisms. Following, exposure assays with model organism and PAH quinone, 1,2-naphthoquinone were conducted. DNA adducts were identified. Possible exposure-specific adducts of guanine were detected. Considering these results, in vitro reactions of 1,2-naphthoquinone and 2’-deoxyguanosine dG were conducted. Products were analyzed by LC-MS/MS to aid in identification of adducts in bacterial DNA. Research is ongoing.
|
| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Model organism was decided and production of PAH-type catechols during biotransformation of PAHs was confirmed by LC/ESI-MS/MS. Genes involved in catabolism of PAHs by this organism were determined and pathways for the biotransformation of model PAHs were mapped. RT-PCR analyses were conducted as part of methods development. Assessment of DNA damage by LC/ESI-MS/MS DNA adductomics was conducted using the model organism and model toxicant hydrogen peroxide. Afterwards, DNA damage assessment was conducted by using 1,2-naphthoquinone. DNA adducts were identified in both conditions. Exposure specific adduct(s) of guanine appeared to have occurred after exposure to 1,2-naphthoquinone. In vitro reactions of 1,2-naphthoquinone and 2’-deoxyguanosine dG were conducted with the aim to identify DNA adducts in bacterial DNA.
|
| Strategy for Future Research Activity |
Continue DNA damage analyses using 1,2-naphthoquinone. Develop methods for conducting transcriptomics on the model organism. Conduct transcriptome analyses. Develop methods for detecting ROS by fluorescence in the model organism.
|
| Causes of Carryover |
Budget from 2015-16 was carried over to 2016-17 because it was sufficient for the experiments conducted in 2015-16.
|
| Expenditure Plan for Carryover Budget |
Continuation of exposure assays by using LC/ESI-MS/MS. Transcriptomics analyses. Presentation of results at International peer review conference.
|
-
[Journal Article] Analysis of the Fine-Tuning of Cyanobacterial Circadian Phase by Monochromatic2016
Author(s)
Kobayashi T, Obana Y, Kuboi N, Kitayama Y, Hayashi S, Oka M, Wada N, Arita K, Shimizu T, Sato M, Kanaly RA, Kutsuna S
-
Journal Title
Plant Cell Physiol
Volume: 57
Pages: 105-114
DOI
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
[Journal Article] MytiLec, a mussel R-type lectin, interacts with surface glycan Gb3 on Burkitt’s lymphoma cells to trigger apoptosis through multiple pathways2015
Author(s)
Imtiaj Hasan, Shigeki Sugawara, Yuki Fujii, Yasuhiro Koide, Daiki Terada, Naoya Iimura, Toshiyuki Fujiwara, Keisuke G. Takahashi, Nobuhiko Kojima, Sultana Rajia, Sarkar M. A. Kawsar, Robert A. Kanaly, Hideho Uchiyama, Masahiro Hosono, Yukiko Ogawa, Hideaki Fujita, Jiharu Hamako, Taei Matsui, Yasuhiro Ozeki
-
Journal Title
Marine Drugs
Volume: 13
Pages: 7377-7389
DOI
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
[Presentation] MytiLec, an R-type lectin from Mediterranean mussel regulates cancer cell growth through glycan-dependent signaling.2015
Author(s)
Hasan I, Fujii Y, Ogawa Y, Sugawara S, Hosono M, Koide Y, Rajia S, Kawsar S M A, Kanaly R A, Fujiwara T, Fujita H, Ozeki Y
Organizer
23rd International Glycoconjugates Symposium (GLYCO23)
Place of Presentation
Split, Croatia
Year and Date
2015-09-15 – 2015-09-20
Int'l Joint Research
-
-
[Book] Chapter 33, "Structural glycobiology for lectin to promote advanced biomedical research" Marine Glycobiology, Principles and Applications. (Edited by Se-Kwon Kim, ISBN: 9781498709613)2016
Author(s)
1. Hasan I, Fujii Y, Rajia S, Kawsar S M A, Sugawara S, Ogawa Y, Hosono M, Koide Y, Kanaly R A, Ozeki Y.
Total Pages
443-458
Publisher
Taylor & Francis Group, CRC press
-
[Book] Chapter 21: SUEL/RBL-Lectins and their biomedical applications" Marine Omics, Principles and Applications (Se-Kwon Kim ed, ISBN 9781482258202)2016
Author(s)
5. Koide Y, Hasan I, Fujii Y, Kawsar SMA, Rajia S, Sugawara S, Ogawa Y, Hosono M, Kanaly RA, Ozeki Y.
Total Pages
407-418
Publisher
Taylor & Francis Group, CRC press
