環境保全微生物に生じたDNAダメージと修復のアダクトミクスに関する研究

2016 Fiscal Year Research-status Report

• Project/Area Number: 26505010
• Research Institution: Yokohama City University
• Principal Investigator: Robert Kanaly 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (00448656)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: DNA adductomics

Outline of Annual Research Achievements

Exposure assays using hydrogen peroxide and 1,2naphthoquinone were conducted. Some putative DNA adducts detected in bacterial cells were identified as DNA adducts by different techniques. Relative levels of known and unknown DNA adducts were compared. Methods were developed for conducting ROS assays. Initial methods for conducting RNA transcriptome analyses were explored.

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

Some putative DNA adducts were identified but require further confirmation. ROS assay assays showed that ROS levels were related to exposure scenarios. Using bacteria, DNA adductomics system was established and has allowed for wider application using human cell lines.

Strategy for Future Research Activity

Complete RT-PCR analyses, confirm unknown adducts, and conduct RNA transcriptomics.

Causes of Carryover

Budget was carried over for the purpose to complete transcriptome analyses.

Expenditure Plan for Carryover Budget

Plan to conduct transcriptome analyses.

Research Products

• [Journal Article] Internalization of a novel, huge lectin from Ibacus novemdentatus (slipper lobster) induces apoptosis of mammalian cancer cells (2017)
  • Author(s): Fujii, Y., T. Fujiwara, Y. Koide, I. Hasan, S. Sugawara, S. Rajia, S. M. A. Kawsar, D. Yamamoto, D. Araki, R. A. Kanaly, Y. Ogawa, H. Fujita, and Y. Ozeki
  • Journal Title: Glyconjugate Journal Volume: 34 Pages: 85-94
  • DOI: 10.1007/s10719-016-9731-x
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] RAMA casein zymography: time-saving and highly sensitive casein zymography for MMP7 and trypsin (2016)
  • Author(s): Yasumitsu, H., Y. Ozeki, and R. A. Kanaly
  • Journal Title: Electrophoresis Volume: 37 Pages: 2959-2962
  • DOI: 10.1002/elps.201600346
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Analysis of the fine-tuning of cyanobacterial circadian phase by monochromatic light and long-day conditions (2016)
  • Author(s): Kobayashi, T., Y. Obana, N. Kuboi, Y. Kitayama, S. Hayashi, M. Oka, N. Wada, K. Arita, T. Shimizu, M. Sato, R.A. Kanaly and S. Kutsuna
  • Journal Title: Plant and Cell Physiology Volume: 57 Pages: 105-114
  • DOI: 10.1093/pcp/pcv177
  • Int'l Joint Research
• [Presentation] Biodegradation of high concentrations of model fuel hydrocarbon NAPLs by a bacterium isolated from soil (2016)
  • Author(s): Takeshita, T., and R. A. Kanaly
  • Organizer: The 31st Annual Meeting of the Japanese Society of Microbial Ecology
  • Place of Presentation: Yokosuka, Kanagawa, Japan
  • Year and Date: 2016-10-22 – 2016-10-25
  • Int'l Joint Research
• [Presentation] Bacterial biodegradation of sulfur-containing aromatic pollutants and the production of disulfides measured by LC-ESI-MS2 (2016)
  • Author(s): Ozawa, K., Nemoto, Y., Kikuchi, M., and R. A. Kanaly
  • Organizer: The 31st Annual Meeting of the Japanese Society of Microbial Ecology
  • Place of Presentation: Yokosuka, Kanagawa, Japan
  • Year and Date: 2016-10-22 – 2016-10-25
  • Int'l Joint Research
• [Presentation] Biodegradation of benzo[a]pyrene by Sphingobium barthaii KK22 and effects on co-occurring PAH biodegradation (2016)
  • Author(s): Izawa, M., and R.A. Kanaly
  • Organizer: The 31st Annual Meeting of the Japanese Society of Microbial Ecology
  • Place of Presentation: Yokosuka, Kanagawa, Japan
  • Year and Date: 2016-10-22 – 2016-10-25
  • Int'l Joint Research
• [Presentation] Chemical and genetic analyses of polycyclic aromatic hydrocarbon biodegradation by Sphingobium barthaii KK22 (2016)
  • Author(s): Maeda, A.H., S. Nishi, Y. Hatada, Y. Ohta, Y. Ozeki, M. Kunihiro and R.A. Kanaly
  • Organizer: The 31st Annual Meeting of the Japanese Society of Microbial Ecology
  • Place of Presentation: Yokosuka, Kanagawa, Japan
  • Year and Date: 2016-10-22 – 2016-10-25
  • Int'l Joint Research
• [Presentation] Biotransformation of PAHs by Sphingobium barthaii and investigation of DNA damage by LC/ESI-MS/MS (2016)
  • Author(s): Fukuoka, K., and R.A. Kanaly
  • Organizer: ASM Microbe 2016
  • Place of Presentation: Boston, MA, USA
  • Year and Date: 2016-06-16 – 2016-06-20
  • Int'l Joint Research