Study on DNA adductomics related to DNA damage and repair involving environmental microorganisms

2017 Fiscal Year Final Research Report

• Project/Area Number: 26505010
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Yokohama City University
• Principal Investigator: Kanaly Robert 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (00448656)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: DNAアダクトミクス / 多環芳香族炭化水素 / Biotransformation

Outline of Final Research Achievements

Bacteria that biodegrade genotoxicants such as polycyclic aromatic hydrocarbons (PAHs) play important roles in carbon cycling of environmental pollutants. During PAH biodegradation, metabolite production may cause DNA damage and mutations which have implications for bacterial evolution. Biodegradation and exposure experiments were conducted with Sphingobium barthaii KK22. PAH-catechol metabolites were produced during biotransformation and gene expression related to PAH-catechol production was confirmed by RT-PCR. DNA adducts, including a PAH-catechol adduct were identified through DNA damage assessment by LC/ESI-MS/MS DNA adductomics and provided first direct evidence that bacteria are subjected to bulky DNA damage during PAH exposure. Increased levels of reactive oxygen species and oxidative DNA adducts were confirmed. RNA transcriptome analyses revealed that more than 600 genes were differentially expressed from PAH-catechol exposure including genes involved in DNA damage repair.

Free Research Field

環境毒性学