New Methods for Mass Spectrometry Imaging using SALDI and ESI Ionization

2016 Fiscal Year Final Research Report

• Project/Area Number: 26505011
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Kansai University
• Principal Investigator: Arakawa Ryuichi 関西大学, 化学生命工学部, 教授 (00127177)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 白金スパッタ蒸着 / レーザー脱離イオン化 / タッピング走査プローブ / エレクトロスプレー / イメージング質量分析 / 葉の農薬分布 / ナノピペット

Outline of Final Research Achievements

The important requirements for mass spectrometry (MS) imaging include achieving desorption/ionization of target samples on a surface as locally as possible. Matrix-assisted laser desorption/ionization (MALDI) has been applied as a powerful ionization technique for MS imaging. However, since MALDI is operated under vacuum conditions, the sample preparation is less convenient for MS imaging. Recently, a number of ambient ionization methods such as desorption electrospray ionization (DESI) have been developed to allow MS imaging for the direct examination of unmodified samples. Here, we demonstrate new methods for MS imaging; one is platinum vapor deposition surface-assisted laser desorption/ionization (Pt-SALDI) and the other is tapping-mode scanning probe electrospray ionization (t-SPESI). These two methods were compared with the conventional methods of MALDI-MS and ambient ESI-MS to be evaluated in terms of simplicity of preparation and spatial resolution.

Free Research Field

質量分析化学