Establishment of molecular basis for skeletal development in medaka

2017 Fiscal Year Final Research Report

• Project/Area Number: 26506005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 宇宙生命学
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Inohaya Keiji 東京工業大学, 生命理工学院, 助教 (70302958)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 発生・分化 / 骨形成 / 骨芽細胞 / メダカ

Outline of Final Research Achievements

sp7/osterix is a zinc finger transcription factor that is essential for osteoblast differentiation in mammals. To verify the characteristic features of osteoblast-lineage cells in teleosts, medaka sp7 mutants were established using a transcription activator-like effector nuclease (TALEN) genome editing system. These mutants showed severe defects in the formation of skeletal structures, indicating that sp7/osterix gene is essential for skeletal development in medaka. In addition, the present study revealed that type X collagen a1 a (col10a1a)-positive cells are osteoblast-like cells and sp7/osterix gene expression in osteoblast-lineage cells is required for differentiation into mature osteoblasts to form the skeletal structures.

Free Research Field

発生生物学