2016 Fiscal Year Final Research Report
Development of nitrogen supply system to agricultural soil using cellulosic plant biomass and nitrogen-fixing bacteria
Project/Area Number |
26520309
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 特設分野 |
Research Field |
Food Cycle Research
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Research Institution | Kagoshima University |
Principal Investigator |
SAKAI Masao 鹿児島大学, 農水産獣医学域農学系, 教授 (20225775)
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Research Collaborator |
KAWAUCHI Tomohiro
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Project Period (FY) |
2014-07-18 – 2017-03-31
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Keywords | 窒素固定 / セルロース分解 / 植物遺体 / 細菌集団 / 集積培養 |
Outline of Final Research Achievements |
We constructed the stable bacterial enrichment culture with functions of cellulose degradation and nitrogen fixation from cut grass residues. When a filter paper was added to the culture, paper degradation was observed within 6 days while simultaneously detecting the nitrogen fixation. The extract DNA from the culture was used for the community analysis. The next generation sequencing (NGS) of 16S rRNA genes showed that enrichment culture contained 11 genera as predominant bacterial groups included both nitrogen fixing and cellulose degrading bacteria. Based on the NGS information, we isolated five nitrogen-fixing bacteria, which sequences are corresponded with the major bacterial groups. The isolates were closely affiliated with genera Azoarcus, Pseudomonas (Azomonas), Azospira, Azospirillum and Rhizobium. They are commonly known as endophytic or symbiotic nitrogen-fixing bacteria. Thus, these nitrogen-fixing bacteria suggested to be key member in this bacterial consortia.
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Free Research Field |
土壌微生物学
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