Construction of an intelligent reaction system for molecular cancer diagnosis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26540151
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Life / Health / Medical informatics
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Komiya Ken 東京工業大学, 情報理工学院, 助教 (20396790)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: DNAコンピュータ / DNAナノテクノロジー / 核酸増幅法 / 分子状態機械 / 早期診断

Outline of Final Research Achievements

In this study, we constructed and investigated an intelligent reaction system for allowing molecules to process information of transcription pattern and conduct cancer diagnosis. The present reaction system consists of a nucleic acid amplification method and a molecular state machine. In the development of nucleic acid amplification method, we achieved suppression of undesired non-specific amplification, improved reaction specificity and extended variation of detectable forms of nucleic acids. Finally, we improved the sensitivity of detection. In the development of molecular state machine, we revealed factors that affect the reaction efficiency and revised the design of reaction system. Optimization of reaction conditions is continuously implemented.

Free Research Field

DNAコンピューティング