Molecular mechanisms of multiple regulation of post-replication repair pathway by deubiquitinases

2015 Fiscal Year Final Research Report

• Project/Area Number: 26550024
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Nagoya University
• Principal Investigator: Masuda Yuji 名古屋大学, 医学系研究科(環医), 准教授 (30273866)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: DNA損傷トレランス

Outline of Final Research Achievements

In humans, cells have two damage tolerance pathways. One is the error-prone pathway, translesion DNA synthesis (TLS). The other is the error-free, in principle, pathway, template switch (TS). These are regulated by ubiquitination of PCNA. Since mono- and poly-ubiquitination of PCNA stimulates TLS and TS, respectively, the regulation of pathway choice is a crucial step for the maintenance of genetic stability. However, in humans, the regulatory mechanism is obscure because poly-ubiquitinated PCNA is only slightly detectable. In this study, we identified deubiquitinases for ubiquitinated PCNA and analyzed their function in the damage tolerance pathways.

Free Research Field

生化学