2015 Fiscal Year Final Research Report
Molecular mechanisms of multiple regulation of post-replication repair pathway by deubiquitinases
Project/Area Number |
26550024
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Risk sciences of radiation and chemicals
|
Research Institution | Nagoya University |
Principal Investigator |
Masuda Yuji 名古屋大学, 医学系研究科(環医), 准教授 (30273866)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Keywords | DNA損傷トレランス |
Outline of Final Research Achievements |
In humans, cells have two damage tolerance pathways. One is the error-prone pathway, translesion DNA synthesis (TLS). The other is the error-free, in principle, pathway, template switch (TS). These are regulated by ubiquitination of PCNA. Since mono- and poly-ubiquitination of PCNA stimulates TLS and TS, respectively, the regulation of pathway choice is a crucial step for the maintenance of genetic stability. However, in humans, the regulatory mechanism is obscure because poly-ubiquitinated PCNA is only slightly detectable. In this study, we identified deubiquitinases for ubiquitinated PCNA and analyzed their function in the damage tolerance pathways.
|
Free Research Field |
生化学
|