2017 Fiscal Year Final Research Report
Evaluation of biological effects induced by ionizing radiation and stress using DNA damage foci formation visualized in living hippocampus neuron and retinal ganglion cells.
| Project/Area Number |
26550029
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Tohoku Medical and Pharmaceutical University (2016-2017)
Tottori University (2014-2015) |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Keywords | DNA損傷 / 放射線 / DNA損傷応答 / 53BP1 / 海馬 / 神経細胞 / タイムラプス / 網膜 |
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| Outline of Final Research Achievements |
Ionizing radiation and inside or outside stress induced in our body result in a increased ROS production and a induction of DNA double-strand breaks in the cells. A hypoxia caused by cerebral ischemia or glaucoma, nuclear foci formation by DNA double-strand breaks is also reported. A sensor of DNA damage foci formation using EGFP-53BP1 fusion protein makes DNA damage formation visible in the living mouse neuronal tissues or cells. As a result of our research, there is a clear difference of foci formation between differentiated tissue in vivo and culture cells in vitro, suggesting there exist special characteristics of foci formation and DNA damage response in the differentiated tissue cells.
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| Free Research Field |
放射線影響
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