2015 Fiscal Year Final Research Report
Construction of DNA damage-specific endonuclease using monoclonal antibody against DNA damage
| Project/Area Number |
26550033
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
森 俊雄 奈良県立医科大学, 医学部, 研究教授 (10115280)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 修復 / DNA 損傷 / シクロブタン型ダイマー / モノクローナル抗体 |
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| Outline of Final Research Achievements |
We succeeded in produing CPD specific endonuclease based on an anti-CPD monoclonal antibody. U2OS expressing this endonuclease got UV resistant. Moreover, time course quantification of CPDs by ELISA after UV irradiation revealed that U2OS expressing the endonuclease removed CPDs more quickly and efficiently compared with control U2OS.
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| Free Research Field |
分子生物学
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