Novel mutagenic pathway involving DNA polymesase

2017 Fiscal Year Final Research Report

• Project/Area Number: 26550034
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: National Institutes for Quantum and Radiological Science and Technology (2016-2017); Japan Atomic Energy Agency (2014-2015)
• Principal Investigator: Shikazono Naoya 国立研究開発法人量子科学技術研究開発機構, 関西光科学研究所 量子生命科学研究部, 部長(定常) (10354961)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: クラスターDNA損傷 / 変異メカニズム

Outline of Final Research Achievements

In this study, in order to gain insight into the unknown mechanism of cluster DNA damage-induced mutagenesis, we investigated whether DNA strand with mutagenic DNA damage is preferentially replicated by DNA polymerase(s). We developed a new method for constructing plasmids that can determine the fraction of DNA strand used as the template for replication in plasmids carrying cluster DNA damage. Using plasmids constructed by the method developed in this study, we found that (1) one of the strand is preferentially replicated around cluster DNA damage, and (2) several tens of base pairs in length are preferentially replicated, and (3) the preferential replication is mediated by PolI.

Free Research Field

放射線生物学