2015 Fiscal Year Final Research Report
Organic arsenicals induced cerebellar symptoms and disturbance of miRNA regulation
| Project/Area Number |
26550043
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Risk sciences of radiation and chemicals
|
|---|
| Research Institution | Meijo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 有機ヒ素化合物 / 酸化ストレス / miRNA / 小脳症状 / アストロサイト / ラット |
|---|
| Outline of Final Research Achievements |
In the arsenic poisoning occurred in Kamisu, Ibaraki, Japan, people drinking highly arsenic-contaminated water of the well suffered from neurological, mainly cerebellar, symptoms. Exposure to diphenylarsinic acid (DPAA), a compound detected in the Kamisu accident, was able to induce abnormal behaviors in rats in vivo and activation of astrocytes (cell proliferation, activation of MAP kinase and transcription factors, and release of MCP-1, adrenomedullin, CXCL1, and IL-6). Here, the role of miRNA, which negatively regulates transcription of these cytokines, miR-181, was investigated. DPAA, which was a main and most active organic arsenicals among those found in the Kamisu accident, reduced miR-181 expression at 10 µM after 96 hour exposure in cultured astrocytes. However, interference using miRNA mimic and miRNA inhibitor specific for miR-181 showed little effect. It seemed that reduction of miR-181 hardly account for DPAA-induced activation of astrocytes.
|
| Free Research Field |
神経毒性学
|
