Introduction of nanoparticles into yeast cells and depelopment of the control technology

2015 Fiscal Year Final Research Report

• Project/Area Number: 26550065
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental engineering and reduction of environmental burden
• Research Institution: Osaka Prefecture University
• Principal Investigator: Nomura Toshiyuki 大阪府立大学, 工学(系)研究科(研究院), 准教授 (00285305)
• Co-Investigator(Kenkyū-buntansha): TANI SHUJI 大阪府立大学, 生命環境科学研究科, 講師 (80405357)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ナノ粒子 / 酵母 / エンドサイトーシス

Outline of Final Research Achievements

We examined the introduction of nanoparticles into cells equipped with a rigid cell wall. Yeast cells were used as model microbial cells, and the positively charged polystyrene latex (PSL) nanoparticles were used as model nanoparticles. As a result, we successfully demonstrated that the introduction of the PSL nanoparticles into the living yeast cells could be promoted using anionic carboxymethylcellulose. In addition, the RNA-Seq analysis of the yeast cells which took in the PSL nanoparticles were performed. As a result, it was suggested that the cells were subjected to stress from the nanoparticles.

Free Research Field

微粒子工学