Resistant Mechanism of Pollutants in Marine Organisms Suffered from Environmental Pollution by the Great East Japan Earthquake

2016 Fiscal Year Annual Research Report

• Project/Area Number: 26550083
• Research Institution: Prefectural University of Kumamoto
• Principal Investigator: 阿草 哲郎 熊本県立大学, 環境共生学部, 准教授 (50403853)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 集団遺伝学的影響 / 生体毒性 / 東日本大震災 / 重油汚染 / PAH / メタゲノム

Outline of Annual Research Achievements

東北地方太平洋沖地震・東日本大震災がもたらした化学物質による海洋生態系への影響アセスメントは重要な課題である。本申請課題では、震災によって深刻な重油汚染が生じた宮城県気仙沼湾の海洋生態系を対象に、重油由来でかつ毒性の高い多環芳香族炭化水素（PAHs）が海洋生態系にどのような集団遺伝学的影響を及ぼしたのか？、つまり、PAHs耐性をもたらしたのか？、という点について明らかにする。
本年度は、まず2016年11月に、気仙沼湾において堆積物12点、アイナメ10匹をそれぞれ採取した。アイナメに関しては、肝臓・生殖腺・血液（血漿）をそれぞれ分取し、液体窒素（-170℃）の入った専用容器に入れ、凍結保存した。その他の試料は、-20℃で冷凍保存した。今回と2011年・2012年・2015年に採取した堆積物に関してDNAを抽出した後、メタゲノムシークエンスを実施し、海底堆積物中菌叢の違いを空間的かつ時間的に解析した。その結果、石油耐性・分解菌を考えられるRhodococcus属は、相対的にPAHs汚染が高いところで多い傾向を示した。また、2011年から経時的にみると、Rhodococcus属は減少傾向にあった。このことから、PAHs汚染が著しいときにRhodococcus属が集まり、徐々にPAHsを分解していったことでPAHs汚染が低減し、その結果Rhodococcus属も減少していったことが予想された。

Research Products

• [Journal Article] Strain differences in the proteome of dioxin-sensitive and -resistant mice treated with 2,3,7,8-tetrabromodibenzo-p-dioxin (2017)
  • Author(s): Nguyen, H.T., Lauan Tsuchiya, M.C., Yoo, J., Iida, M., Agusa, T., Hirano, M., Kim, E.-Y., Miyazaki, T., Nose, M., Iwata, H.
  • Journal Title: Archives of Toxicology Volume: 4 Pages: 1763-1782
  • DOI: doi: 10.1007/s00204-016-1834-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Lead contamination in surface soil on roads from used lead acid battery recycling in Northern Vietnam (2016)
  • Author(s): Fujimori, T., Eguchi, A., Agusa, T., Tue, N.M., Suzuki, G., Takahashi, S., Viet, P.H., Tanabe, S., Takigami, H.
  • Journal Title: Journal of Material Cycles and Waste Management Volume: 18 Pages: 599-607
  • DOI: 10.1007/s10163-016-0527-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of aryl hydrocarbon receptor signaling pathways altered in TCDD-treated red seabream embryos by transcriptome analysis (2016)
  • Author(s): Iida, M., Fujii, S., Uchida, M., Nakamura, H., Kagami, Y., Agusa, T., Hirano, M., Bak, S.-M., Kim, E.-Y., Iwata, H.
  • Journal Title: Aquatic Toxicology Volume: 177 Pages: 156-170
  • DOI: 10.1016/j.aquatox.2016.05.014
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Arsenic Pollution in Southeast Asia (2017)
  • Author(s): Agusa, T., Nakata, H., Iwata, H., Tanabe, S.
  • Organizer: Japan - Taiwan International Symposium on Environmental Science, Technology and Management
  • Place of Presentation: 北九州
  • Year and Date: 2017-03-30 – 2017-03-31
  • Int'l Joint Research / Invited
• [Presentation] Water pollution by trace elements in Myanmar (2016)
  • Author(s): Murakami, R., Omagari, R., Uchiyama, Y., Nakata, H., Phay, N., Agusa, T.
  • Organizer: Third Myanmar-Japan International Symposium
  • Place of Presentation: Pathein, Myanmar
  • Year and Date: 2016-12-03 – 2016-12-04
  • Int'l Joint Research
• [Presentation] Trace elements in groundwater after The 2016 Kumamoto Earthquakes (2016)
  • Author(s): Kimura, N., Murakami, R., Ishii, E., Hashioto, M., Uchiyama, Y., Hosono, T., Nakata, H., Agusa, T.
  • Organizer: Third Myanmar-Japan International Symposium
  • Place of Presentation: Pathein, Myanmar
  • Year and Date: 2016-12-03 – 2016-12-04
  • Int'l Joint Research
• [Presentation] メタボローム解析を用いたベトナムの地下水ヒ素汚染地域住民の影響評価：第2報 (2016)
  • Author(s): 阿草哲郎, 江口哲史, 國頭 恭, Tue, N.M., Lan, V.T.M., Minh, T.B., Trang, P.T.K., 高橋 真, Viet, P.H., 田辺信介, 岩田久人
  • Organizer: 第22回ヒ素シンポジウム
  • Place of Presentation: 江東区
  • Year and Date: 2016-11-17 – 2016-11-18
  • Int'l Joint Research
• [Presentation] Contamination by trace elements associated with e-waste recycling processes in developing countries (2016)
  • Author(s): Agusa, T., Takigami, H., Fujimori, T., Eguchi, A., Bekki, K., Yoshida, A., Terazono, A., Ha, N.N., Asante, K.A., Biney, C.A., Agyekum, W.A., Bello, M., Ramu, K., Ballesteros Jr., F.C., Bulbule, K.A., Parthasaraty, P., Takahashi, S., Subramanian, A., Iwata, H., Tanabe, S.
  • Organizer: 2016 1st Eastern Asia Conference for Sustainable Economy (EACSE)
  • Place of Presentation: Taoyuan, Taiwan
  • Year and Date: 2016-09-26
  • Int'l Joint Research
• [Presentation] Mercury pollution at small-scale gold mining and e-waste recycling areas in Indonesia (2016)
  • Author(s): Hayama, S., Uchiyama, Y., Sudaryanto, A., Yoshida, A., Oguchi, M., Terazono, A., Skandarl, Tanabe, A., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science
  • Place of Presentation: Taoyuan, Taiwan
  • Year and Date: 2016-09-26
  • Int'l Joint Research
• [Presentation] Urgent survey on groundwater pollution caused by The 2016 Kumamoto Earthquakes (2016)
  • Author(s): Kimura, N., Murakami, R., Ishii, E., Hashioto, M., Uchiyama, Y., Hosono, T., Nakata, H., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science
  • Place of Presentation: Taoyuan, Taiwan
  • Year and Date: 2016-09-26
  • Int'l Joint Research
• [Presentation] Chemical pollution in water environment in Myanmar (2016)
  • Author(s): Murakami, R., Omagari, R., Uchiyama, Y., Nakata, H., Phay, N., Agusa, T.
  • Organizer: Taiwan-Japan International Symposium on Environmental Science
  • Place of Presentation: Taoyuan, Taiwan
  • Year and Date: 2016-09-26
  • Int'l Joint Research
• [Presentation] ベトナムの地下水ヒ素汚染とヒトへの曝露 (2016)
  • Author(s): 阿草哲郎
  • Organizer: 環境毒性化学とメダカに関する研究会 around 九州
  • Place of Presentation: 熊本
  • Year and Date: 2016-09-12
  • Int'l Joint Research
• [Presentation] メタボロミクスを用いたヒ素汚染地域住民の影響評価 (2016)
  • Author(s): 阿草哲郎, 江口哲史, Tue, N.M., Lan, V.T.M., Minh, T.B., Trang, P.T.K., 高橋 真, Viet, P.H., 田辺信介, 岩田久人
  • Organizer: 第25回環境化学討論会
  • Place of Presentation: 新潟
  • Year and Date: 2016-06-08 – 2016-06-10
  • Int'l Joint Research