2016 Fiscal Year Final Research Report
Construction of a plant biomass-degrading recombinant anaerobic bacterium and its utilization for biorefinery
| Project/Area Number |
26550103
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Design and evaluation of sustainable and environmental conscious system
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HIRANO Katsuaki 日本大学, 工学部, 博士研究員 (20723753)
SHINODA Suguru 日本大学, 工学部, 博士研究員 (60747666)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | リグノセルロース / セルロソーム / 糖化発酵 |
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| Outline of Final Research Achievements |
Cellulosome-producing thermophilic anaerobic Clostridia play a pivotal role in the degradation of cellulosic biomass in a thermophilic methanogenic bioreactor. The cellulosome is a supramolecular multienzyme complex composed of a wide variety of polysaccharide-degrading enzymes and structural proteins, and displayed on the cell surface of anaerobic cellulolytic bacteria. Cellulosomal enzymes bound to the structural proteins generate synergy for the degradation of crystalline substrates, such as crystalline cellulose and plant biomass. In this study, we constructed a recombinant thermophilic anaerobic bacterium, which produces enzymes that are not naturally present in the cellulosome complex, beneficial to the fermentation of renewable bio-based fuels and chemicals.
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| Free Research Field |
微生物工学
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