2015 Fiscal Year Final Research Report
Quantification of hemolytic conditions using pico-photo sensing of chemoluminescence caused by hemoglobin leakage
| Project/Area Number |
26560201
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Saitama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UJIHARA Yoshihiro 川崎医科大学, 医学部, 助教 (80610021)
KIMURA Naoyuki 自治医科大学, 医学部, 講師 (20382898)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 溶血 / 赤血球 / 引張り試験 |
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| Outline of Final Research Achievements |
1) Fracture strain of a red blood cell (RBC): Tensile tests of a RBC were implemented under a microscope. The fracture strain was mostly ranged from 3 to 4, and was 5.32 at maximum.
2) Effects of the hold position on RBC strain behavior during tensile tests: Numerical examinations of tensile tests revealed that significant variations in the deformed geometry of RBC during the tensile test, as well as variations in strain distribution. Of the hold patterns examined, with an applied strain of 0.8, the misaligned stretch increased the maximum of the first principal strain by 65-85% in comparison to the aligned stretch.
3) Visualization of hemolysis: Luminol reaction was utilized to visualize the occurrence of hemolysis. A hypotonic luminol solution was then injected to the channel where RBCs were placed. An EM-CCD camera showed strips of light extending downstream from the positions where the RBCs had been, indicating the success of capturing the instance of hemolysis at a RBC scale.
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| Free Research Field |
バイオメカニクス
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