Hair follicle tissue engineering by fully-in vitro process using multi-layered gel bead culture

2015 Fiscal Year Final Research Report

• Project/Area Number: 26560222
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Keio University
• Principal Investigator: Miyata Shogo 慶應義塾大学, 理工学部, 准教授 (70376515)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 毛髪再生 / 生体組織工学 / 三次元培養 / 共培養 / 再生医療

Outline of Final Research Achievements

The purpose of this study is to regenerate hair tissue in vitro by controlling three-dimensional cellular organization. To simulate hair follicle structure, collagen gel including two kinds of cells was multi-layered to construct three-dimensional structure.
In this study, mouse embryonic stem cells (mESCs) suspended in collagen gel were micro-spotted and over-layered by mouse fatal epidermal cells suspended in collagen. After the gel beads spotting, cell-seeded collagen gels were cultured in vitro. As the results, hair follicle was regenerated in vitro using our novel culture method. Moreover, arrector pili muscle-like tissue was also regenerated.
In conclusion, three-dimensional culture method simulating hair follicle structure was newly developed. Using our novel method, micro-spotted gel-cultured specimen indicated capability to regenerate follicle by fully-in vitro culture process.

Free Research Field

再生医工学