Development of core technologies for heart diagnostic imaging apparatus based on nanoanalysis

2017 Fiscal Year Final Research Report

• Project/Area Number: 26560225
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Jikei University School of Medicine
• Principal Investigator: Fukuda Norio 東京慈恵会医科大学, 医学部, 准教授 (30301534)
• Co-Investigator(Kenkyū-buntansha): 照井 貴子 東京慈恵会医科大学, 医学部, 助手 (10366247) ; 小比類巻 生 東京慈恵会医科大学, 医学部, 助教 (40548905) ; 大山 廣太郎 東京慈恵会医科大学, 医学部, 特別研究員 (70632131)
• Co-Investigator(Renkei-kenkyūsha): ISHIWATA SHIN'ICHI 早稲田大学, 理工学術院, 名誉教授 (10130866)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: ナノバイオ / 先端機器デバイス / 循環器・高血圧 / 分子イメージング

Outline of Final Research Achievements

1）We developed an experimental system for simultaneous nano-imaging of intracellular Ca dynamics and single sarcomere length in rat neonatal cardiomyocytes. We achieved this by expressing a fluorescence resonance energy transfer-based Ca sensor yellow Cameleon-Nano fused to α-actinin in order to localize to the Z disks. 2）We developed a high-speed (100 fps), high-resolution (20 nm) nano-imaging system for myocardial sarcomeres in living mice. During imaging, we measured macroscopic parameters such as electrocardiogram and left ventricular pressure. 3) We developed a digitally-controlled image reconstruction system for in vivo nano-imaging. Namely, the position of the objective lens was moved downward in the Z-direction during imaging. After experimentation, the LVP record was divided into 17 phases (i.e., 10.2 ms per phase); best-focused images were selected at each phase, and then, combined to obtain an image sequence.

Free Research Field

生理学