2014 Fiscal Year Final Research Report
Development of a novel in vivo imaging technique to visualize renin activity and its application to explore the pathogenesis of hypertensive organ damages.
| Project/Area Number |
26560229
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Minoru 川崎医科大学, 医学部, 准教授 (70449891)
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| Project Period (FY) |
2014-04-01 – 2015-03-31
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| Keywords | 慢性腎臓病 / レニン / FRET / in vivoイメージング / 多光子レーザー顕微鏡 |
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| Outline of Final Research Achievements |
The renin-angiotensin system (RAS) plays an important role in regulating blood volume and systemic vascular resistance. RAS is also deeply implicated in the pathogenesis of wide variety of cardio-vascular and renal diseases. In addition to this circulating RAS, the so-called tissue RAS can act locally as a paracrine and/or autocrine factor. The precise localization of tissue RAS and its regulatory mechanisms have not been fully elucidated. To address this issue, we have applied the technique of fluorescence resonance energy transfer (FRET) and renin FRET peptide. We have successfully developed the technique to detect renin activity in the kidney of living animal. The combination of multiphoton fluorescence imaging of the living kidney with the FRET-based fluorogenic renin substrate provided a novel experimental approach for studying the localization and activity of renin in the intact kidney.
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| Free Research Field |
腎臓病学
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