2015 Fiscal Year Final Research Report
Development of serum-free medium and the establishment of serum-independent cell lines
| Project/Area Number |
26560242
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 血清 / 無血清 / FBS / リポタンパク質 / アルブミン / 有機酸 / T細胞 |
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| Outline of Final Research Achievements |
In this study, it was an objective to identify and profile the protein factors, lipids and organic acids included in the animal serum, participating in the cell proliferation and/or growth inhibition by using T-cell lines. In addition, we planned the improvement of the cell culture method to rely on in 100% import serum (FBS). As a result, it was thought that lipoprotein such as the LDL was effective for the cell proliferation. In addition, the promotional effect was accepted by organic acids including citric acid and pyruvic acid and was able to confirm that the recombinant albumin was also an important promotional factor in the cell culture. Furthermore, after analyzing it, we understood that cholesterol was the most important lipid and was able to prepare a trial product of the low protein serum-free medium by adding in, transferrin, selenious acid and recombinant albumin.
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| Free Research Field |
細胞生物学
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