2015 Fiscal Year Final Research Report
Molecular evolution and functional analysis of influenza virus-binding peptides
| Project/Area Number |
26560245
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | ペプチド / インフルエンザウイルス / ヘマグルチニン / ファージディスプレイ法 / 等温滴定型カロリメーター / 感染阻害剤 / ダイヤモンド電極 / ウイルスセンシング |
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| Outline of Final Research Achievements |
For taking measures against pandemic by emerging of new strains of influenza virus (IFV), we carried out the development of virus detection method and infection inhibitor using IFV-binding peptides. IFV hemagglutinin (HA)-binding peptides were selected from a phage-displayed peptide library. Thermodynamic parameters for the interaction between peptides and HA were determined by isothermal titration calorimetry, and it was indicated that pentapeptides suitable for the size of the receptor-binding pocket in HA showed the similar enthalpy change with pentadecapeptides. The pentapeptide ARLPR showed high infection inhibition activity against major IFV subtypes. Furthermore, by the immobilization of the HA-binding peptides on diamond electrode, we could achieve highly sensitive detection of IFV.
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| Free Research Field |
生命科学
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