クレアチン・アナログ投与がラット骨格筋のエネルギー代謝特性に及ぼす影響

2015 Fiscal Year Annual Research Report

• Project/Area Number: 26560373
• Research Institution: Doshisha University
• Principal Investigator: 大平 充宣 同志社大学, スポーツ健康科学部, 教授 (50185378)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ラット骨格筋 / クレアチン枯渇 / 炭水化物代謝 / 脂質代謝

Outline of Annual Research Achievements

骨格筋の収縮・代謝特性が、高エネルギーリン酸含有レベルにどのような影響を受けるのか、ラットを使って追求した。筋中高エネルギーリン酸含有量は、クレアチンまたはそのアナログであるbeta-guanidinopropionic acid (beta-GPA) の経口投与によって調節し、麻酔下での大腿部坐骨神経を介した右ヒラメ筋または左長指伸筋のin situ電気刺激に対する収縮特性と炭水化物および脂質代謝を追求した。その結果、特に長指伸筋で顕著であったが、ヒラメ筋にもbeta-GPA投与に伴う更なる遅筋化が見られた。また、5分間のtrain stimulation (10 Hz)直後の筋で分析した結果、コントロール食ラットでは顕著な乳酸の増加とクエン酸の減少が見られた。しかし、beta-GPA投与群ではマイナーであり、炭水化物代謝の抑制と脂質代謝の亢進が示唆された。また、ミトコンドリア酵素の活性化も誘発されており、収縮特性のみならず、ミトコンドリアにおける脂質代謝に改善が起こることが明らかとなった。日常生活における筋の収縮活動レベルは減少しているにも関わらず、長期間のクレアチン枯渇による筋の有酸素能が改善されることを示すもので、基礎科学分野のみならず、運動トレーニング分野においても有意義な示唆を与えてくれるものと思われる。しかし、一時注目されていたクレアチン投与に対しては、顕著な効果は認められなかった。

Research Products

• [Journal Article] Responses of skeletal muscles to gravitational unloading and/or reloading (2015)
  • Author(s): Ohira, T., F. Kawano, T. Ohira, K. Goto, and Y. Ohira
  • Journal Title: J. Physiol. Sci. Volume: 65 Pages: 293-310
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Macrophage deficiency in osteopetrotic (op/op) mice inhibits activation of satellite cells and prevents muscle hypertrophy in single soleus fibers (2015)
  • Author(s): Ohira, T., X.D. Wang, T. Ito, F. Kawano, K. Goto, T. Izawa, H. Ohno, T. Kizaki, and Y. Ohira
  • Journal Title: Am. J. Physiol. Cell Physiol. Volume: 308 (10) Pages: C848-C855
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Speed and/or inclination-dependent mobilization of human leg muscles during walking with a unique exception (2015)
  • Author(s): Ohira, T., H. Okabe, F. Kawano, R. Fujita, T. Ohira, K. Ohira, Y. Oke, N. Nakai, R.R. Roy, V.R. Edgerton, and Y. Ohira
  • Journal Title: Acta Astronautica Volume: 116 Pages: 237-246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of long time exposure to simulated micro- and hypergravity on skeletal architecture (2015)
  • Author(s): Canciani, B., A. Ruggiu, A. Giuliani, D. Panetta, K. Marozzi, M. Tripodi, P.A. Salvadori, M. Cilli, Y. Ohira, R. Cancedda, and S. Tavella
  • Journal Title: J. Mech. Behav. Biomed. Mater. Volume: 51 Pages: 1-12
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Habitual exercise training acts as a physiological stimulator for constant activation of lipolytic enzymes in rat primary white adipocytes (2015)
  • Author(s): Ogasawara, J., T. Izawa, T. Sakurai, K. Shirato, Y. Ishibashi, Y. Ohira, H. Ishida, H. Ohno, and T. Kizaki
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 464 Pages: 348-353
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effect of circadian rhythm on clinical and pathophysiological conditions and inflammation (2015)
  • Author(s): Kizaki, T., S. Sato, K. Shirato, T. Sakurai, J. Ogasawara, T. Izawa, Y. Ohira, K. Suzuki, and H. Ohno.
  • Journal Title: Critical ReviewsTM in Immunology Volume: 35 (4) Pages: 261-275
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Heat stress effects on the myosin heavy chain phenotype of rat soleus fibers during the early stages of regeneration (2015)
  • Author(s): Oishi, Y., R.R. Roy, T. Ogata, and Y. Ohira
  • Journal Title: Muscle & Nerve Volume: 52 Pages: 1047-1056
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 最大酸素摂取量レベルは持久性運動パフォーマンスにどれだけ貢献するのか？ (2015)
  • Author(s): 柴原拓哉、大場友裕、石原達朗、田中剛貴、大平充宣
  • Journal Title: 同志社スポーツ健康科学 Volume: 7 Pages: 52-66
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phosphorylation of Hsp25 at serine 15 is promoted in immature fibers following in situ muscle contraction (2015)
  • Author(s): Kawano, F., T. Shibaguchi, N. Nakai, K. Nakata, and Y. Ohira
  • Journal Title: J. Phys. Fitness Sports Med. Volume: 4 (2) Pages: 231-239
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Deficiency of heat shock transcription factor 1 suppresses heat stress-associated increase in slow soleus muscle mass of mice (2015)
  • Author(s): Ohno, Y., T. Egawa, S. Yokoyama, A. Nakai, T. Sugiura, Y. Ohira, T. Yoshioka, and K. Goto
  • Journal Title: Acta Physiologica Volume: 215 Pages: 191-203
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Differences in histone modifications between slow- and fast-twitch muscle of adult rats and following overload, denervation or valproic acid administration (2015)
  • Author(s): Kawano, F., K. Nimura, S. Ishino, N. Nakai, K. Nakata, and Y. Ohira
  • Journal Title: J. Appl. Physiol. Volume: 119 Pages: 1042-1052
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Involvement of AMPK in regulating skeletal muscle atrophy during hindlimb unloading in mice (2015)
  • Author(s): Egawa, T., A. Goto, Y. Ohno, S. Yokoyama, A. Ikuta, M. Suzuki, T. Sugiura, Y. Ohira, T. Yoshioka, T. Hayashi, and K. Goto
  • Journal Title: Am. J. Physiol. Endocrinol. Metabol. Volume: 309 Pages: E651-E662
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Estimation of leg-muscle mobilization during walking on the Mars and the Moon using an antigravity treadmill in humans (2016)
  • Author(s): Kamibayashi, K., T. Wakahara, S. Yoshida, N. Tsujiuchi, A. Ito, Y. Nakamura, T. Izawa, Y. Fujisawa, and Y. Ohira
  • Organizer: 2016 International Space Station Research & Development (ISSR&D) Cnference
  • Place of Presentation: San Diego, U.S.A.
  • Year and Date: 2016-07-12 – 2016-07-14
  • Int'l Joint Research / Invited
• [Presentation] Effects of afferent inputs, related to the gravity levels, on the physiological and/or biochemical adaptation (2016)
  • Author(s): Ohira, Y.
  • Organizer: Seminar at University of California at San Diego (UCSD) Center for Musculoskeletal Research & Department of Orthopaedic Sugery
  • Place of Presentation: Univ. Calif. San Diego, U.S.A.
  • Year and Date: 2016-07-11
  • Int'l Joint Research / Invited
• [Presentation] Expression level of importin and exportin of mouse skeletal muscle in response to unloading with or without reloading (2015)
  • Author(s): Goto, K., T. Sugiura, Y. Ohira, and T. Yoshioka
  • Organizer: 8th FAOPS (Federation of Asia and Oceania Physiological Society) Congress
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-11-22 – 2015-11-25
  • Int'l Joint Research
• [Presentation] 再生筋におけるエピゲノム変化が過負荷による肥大応答性に及ぼす影響 (2015)
  • Author(s): 河野史倫、小野悠介、藤田諒、中田研、大平充宣、中井直也
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山市
  • Year and Date: 2015-09-18 – 2015-09-20
• [Presentation] 筋損傷後の異なる温度刺激の組み合わせが再生課程における筋衛星細胞の動態に及ぼす影響 (2015)
  • Author(s): 芝口翼、杉浦崇夫、吉原利典、内藤久士、後藤勝正、大平充宣、吉岡利忠
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山市
  • Year and Date: 2015-09-18 – 2015-09-20
• [Presentation] 筋損傷後のアイシングと温熱刺激の組み合わせが回復過程におけるミオシン重鎖分子種に及ぼす影響 (2015)
  • Author(s): 杉浦崇夫、芝口翼、吉原利典、内藤久士、後藤勝正、大平充宣、吉岡利忠
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山市
  • Year and Date: 2015-09-18 – 2015-09-20
• [Presentation] 熱ショック転写因子1はmicroRNAを介した遅筋型ミオシン重鎖発現を抑制する (2015)
  • Author(s): 横山真吾、大野善隆、江川達郎、杉浦崇夫、大平充宣、吉岡利忠、後藤勝正
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山市
  • Year and Date: 2015-09-18 – 2015-09-20
• [Presentation] 乳酸が培養骨格筋細胞の分化に及ぼす影響 (2015)
  • Author(s): 大野善隆、杉浦崇夫、大平充宣、吉岡利忠、後藤勝正
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山市
  • Year and Date: 2015-09-18 – 2015-09-20
• [Presentation] 骨格筋細胞の分化におけるアディポネクチン－アディポネクチン受容体１シグナルの関与 (2015)
  • Author(s): 伊藤理香、横山真吾、大野善隆、江川達郎、宅和美穂、生田旭洋、杉浦崇夫、大平充宣、吉岡利忠、後藤勝正
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山市
  • Year and Date: 2015-09-18 – 2015-09-20
• [Presentation] Involvement of AMPK on hindlimb unloading-induced adaptation of muscle mass in mice (2015)
  • Author(s): Egawa, T., A. Goto, Y. Ohno, S. Yokoyama, M. Suzuki-Takuwa, A. Ikuta, T. Hayashi, T. Sugiura, Y. Ohira, T. Yoshioka, and K. Goto.
  • Organizer: The 11th World Congress of the International Society for Adaptive Medicine
  • Place of Presentation: Yonago City, Japan
  • Year and Date: 2015-05-27 – 2015-05-30
  • Int'l Joint Research
• [Presentation] Suppression of myostatin facilitates the regeneration of injured skeletal muscle in mice under unloading condition (2015)
  • Author(s): Ohno, Y., Y. Matsuba, N. Hashimoto, T. Sugiura, Y. Ohira, T. Yoshioka, and K. Goto
  • Organizer: The 11th World Congress of the International Society for Adaptive Medicine
  • Place of Presentation: Yonago City, Japan
  • Year and Date: 2015-05-27 – 2015-05-30
  • Int'l Joint Research
• [Presentation] Role(s) of antigravitational activity-related afferent input in the regulation of the properties of skeletal muscles and/or immune system. (2015)
  • Author(s): Ohira, Y., F. Kawano, T. Ohira, and Y. Arima
  • Organizer: The 11th World Congress of the International Society for Adaptive Medicine
  • Place of Presentation: Yonago City, Japan
  • Year and Date: 2015-05-27 – 2015-05-30
  • Int'l Joint Research / Invited
• [Presentation] A role of stress response in the regrowth and regeneration of skeletal muscle cells (2015)
  • Author(s): Goto, K., T. Sugiura, Y. Ohira, and T. Yoshioka
  • Organizer: The 11th World Congress of the International Society for Adaptive Medicine
  • Place of Presentation: Yonago City, Japan
  • Year and Date: 2015-05-27 – 2015-05-30
  • Int'l Joint Research / Invited