Elucidation of new functions of alpha actin in skeletal muscle

2016 Fiscal Year Final Research Report

• Project/Area Number: 26560402
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied health science
• Research Institution: Hirosaki Gakuin University
• Principal Investigator: Uda Munehiro 弘前学院大学, 看護学部, 講師 (80549262)
• Co-Investigator(Renkei-kenkyūsha): YAMAKURA Fumiyuki 順天堂大学, 国際教養学部, 特任教授 (20053358) ; BABA Takeshi 順天堂大学, 医学部, 准教授 (80366450)
• Research Collaborator: MATSUMOTO Ayako 順天堂大学, スポーツ健康医科学研究所, 技術員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 骨格筋 / 翻訳後修飾 / アクチン

Outline of Final Research Achievements

In this study, we examined the differences in the protein expression profiles between the soleus and plantaris muscles, and we identified large amount of protein spots at approximately 60 kDa of the soleus muscle compared with the plantaris muscle. These spots were identified as α-skeletal muscle actin by liquid chromatography-nanoelectrospray ionization-tandem mass spectrometry and western blot analyses. In addition, we found that the 60 kDa α-skeletal muscle actin might be modified by small ubiquitin-like modifier (SUMO)-1 protein. Furthermore, we found that α-skeletal muscle actin with larger molecular weight might be localized in the nuclear and cytosol of the skeletal muscle. Finally, we examined the effects of hindlimb unloading on the 60 kDa α-skeletal muscle actin expression in both muscles. We found that hindlimb unloading did not change the expression of 60 kDa α-skeletal muscle actin in both muscles.

Free Research Field

健康・スポーツ科学