In vitro selection of novel functional RNAs and peptides by means of natural RNA library

2017 Fiscal Year Final Research Report

• Project/Area Number: 26560429
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Katoh Takayuki 東京大学, 大学院理学系研究科(理学部), 助教 (90567760)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 機能性ペプチド / 機能性RNA / in vitroセレクション / アゴニスト

Outline of Final Research Achievements

We developed a new method for screening novel functional RNAs and peptides that specifically bind to target molecules in cell. Here, we prepared a total RNA library derived from various human tissues, then mixed them with folate molecules covalently immobilized on magnetic beads to isolate folate-binding RNAs. The RNA library was also subjected to in vitro translation to prepare a peptide library, which was then used to screen Keap1 binding peptides by means of mRNA display.

Free Research Field

分子生物学