Controlling protein/cell biofunction by chemical introduction of glycans

2016 Fiscal Year Final Research Report

• Project/Area Number: 26560438
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Keio University (2016); Institute of Physical and Chemical Research (2014-2015)
• Principal Investigator: Ogura Akihiro 慶應義塾大学, 理工学部(矢上), 助教 (70707843)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 糖鎖 / 糖タンパク質 / ターゲティング / in vivo蛍光イメージング / パターン認識 / 生体内合成

Outline of Final Research Achievements

We have developed an efficient strategy for synthesis of artificial glycoprotein. This strategy provides the target molecule just by mixing the reagent under mild conditions and does not need prior genetic modification. The glycoproteins thus synthesized were injected to mice and their in vivo kinetics was analyzed. It was revealed that excretion pathway could be controlled and cell selective accumulation was achieved depending on the glycan structure on the protein. Furthermore, first organ-selective metal-catalyzed organic reaction was realized by attaching metal catalyst onto this artificial glycoprotein.

Free Research Field

生物分子化学