Development of labeling/purifying/detecting methods using a reactive affinity tag

2016 Fiscal Year Final Research Report

• Project/Area Number: 26560439
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: DODO KOSUKE 国立研究開発法人理化学研究所, 袖岡有機合成化学研究室, 専任研究員 (20415243)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 有機化学 / タンパク質 / ペプチド / アフィニティー精製 / アフィニティーラベリング

Outline of Final Research Achievements

To identify the target proteins and binding sites of bioactive compounds, we planned to develop “reactive affinity tag”, which can label/purify/detect binding proteins of bioactive compounds. As a result, we successfully installed another function into β-ketoamide or O-nitrobenzoxadiazole (NBD) unit. Finally, we developed a reactive affinity tag, which enabled us to label/detect the binding proteins of bioactive compounds and purify/identify the labeled peptides.

Free Research Field

化学反応を利用した生物活性化合物の結合分子同定法の開発