Development of fluorescence-emitting photoaffinity probe that enables the visualization of cross-linking process within cells.

2015 Fiscal Year Final Research Report

• Project/Area Number: 26560449
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: The University of Tokushima
• Principal Investigator: NAMBA Kosuke 徳島大学, 大学院医歯薬学研究部, 教授 (50414123)
• Co-Investigator(Renkei-kenkyūsha): MURATA Yoshiko 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主席研究員 (60256047)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 蛍光分子 / 光親和性標識 / チオールセンサー / 単分子白色発光 / 1,3a,6a-トリアザペンタレン / 蛍光標識

Outline of Final Research Achievements

A white-light-emitting molecule was developed. Since the fluorescence color of this molecule was changed from white to blue by UV irradiation within several minutes, this molecule was suggested to be a useful photoaffinity probe that can detect the labeling process of target protein by the observation of fluorescence color change. Furthermore, development of yellow and red fluorescence compact molecules and novel thiol sensor, substantially increasing the fluorescence intensity by the specific addition of thiol groups, were achieved.

Free Research Field

有機合成化学