2015 Fiscal Year Final Research Report
Quantitative measurements and controlled designs of firefly bioluminescence with genetic bio-technology
| Project/Area Number |
26610081
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Condensed matter physics I
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIYAMA Miyabi 東京大学, 物性研究所, 研究員 (90399311)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 生物発光 / 酵素 / ルシフェラーゼ / ルシフェリン / 分光 |
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| Outline of Final Research Achievements |
We studied quantum yields, spectra, and their dependences on temperatures and solvents, on luciferin-luciferase reactions in firefly bioluminescence, by using biosynthetic mutant luciferase enzyme, where charges, polarity, and hydrophobicity/ hydrophilicity of amino acid residues surrounding oxyluciferin are modified. We also measured quantum yields of biosynthetic mutant photo-protein (aequorin) in jellyfish bioluminescence, by revising our quantitative spectrometer to cover 400 nm blue region. Theoretical analyses via time-dependent density-functional calculations, first-principle calculations, and molecular dynamics calculations including water and protein molecules were made.
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| Free Research Field |
数物系科学
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