2016 Fiscal Year Final Research Report
Thermodynamic study of RNA trinucleotide recognition within silica mesopores
| Project/Area Number |
26620112
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Ibaraki University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | ナノ細孔 / 三塩基認識 |
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| Outline of Final Research Achievements |
In the present study, we aimed to clarify trinucleotide recognition process occurred inside meso-sized cavities of mesoporous silica. As the results, we found following three characteristics in the trinucleotide recognition: (1) efficient duplex formation of short (3- and 4-mer) DNA fragments could be achieved inside silica mesopores under cryogenic conditions, (2) DNA hairpin structure, on the other hand, was destabilized by the confinement inside the mesopores, (3) the duplex stability was significantly enhanced by size-matching between the pore size and the duplex width.
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| Free Research Field |
分析化学
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