Aqueous / aqueous partition utilizing spontaneous emulsification

2015 Fiscal Year Final Research Report

• Project/Area Number: 26620116
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: HIBARA Akihide 東京工業大学, 大学院理工学研究科, 准教授 (30312995)
• Co-Investigator(Kenkyū-buntansha): FUKUYAMA Mao 京都工芸繊維大学, グローバルエクセレンス, 助教 (40754429)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: マイクロ流体 / マイクロ液滴 / ナノ液滴 / 濃縮 / 分離 / タンパク / 界面活性剤

Outline of Final Research Achievements

Water-in-oil microdroplets formed in microfluidic devices have been studied with eagerness in the past decade because of they can be used as picolitter sized chemical containers and applied to analysis for high-throughput microanalyses such as single cell assay. However, the quantification of the microdroplet contents was difficult because of its short optical path lengths. In order to overcome this difficulty, we developed the selective condensation method for microdroplet contents by utilizing spontaneous emulsification at the interface of the microdroplets. Here, we demonstrated the selective condensation of analyte molecules in microspaces, and their applications to protein determination and crystallization.

Free Research Field

分析化学