2015 Fiscal Year Final Research Report
Development of optogenetic tools for controlling kinase activities in insulin signaling pathways and their practical applications
Project/Area Number |
26620128
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
|
Research Institution | The University of Tokyo |
Principal Investigator |
Ozawa Takeaki 東京大学, 理学(系)研究科(研究院), 教授 (40302806)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Keywords | 光制御 / インスリン / キナーゼ |
Outline of Final Research Achievements |
We developed an optogenetic tools for controlling kinase activities of Akt and c-Raf, which are crucial proteins for insulin signaling. Akt was connected with a photo-receptor (CRY2). Upon irradiation with blue light, we observed movement of Foxo1 from nucleus to the cytosol. Moreover, we demonstrated quantitative control of Atrogin-1 gene expression by blue light. Using the same approach, an optogenetic technique to control c-Raf activity in living cells. We demonstrated that red light irradiation enabled to quantitative regulate c-Raf activity. The developed techniques will be useful tools for quantitative analysis of intracellular insulin signaling in living cells.
|
Free Research Field |
分析化学
|