Discovery and application of frequency dependence of microwave-assisted degradation of mucin

2016 Fiscal Year Final Research Report

• Project/Area Number: 26620142
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KAMEYAMA AKIHIKO 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 上級主任研究員 (80415661)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ムチン / ヒアルロン酸 / マイクロ波 / 糖ペプチド

Outline of Final Research Achievements

We have studied a microwave-assisted degradation of mucins that have bound water molecules. MUC2 tandem repeat of 16 mer peptide containing 8 GalNAc residues has been synthesized as a model compound. As a model compound for degradation experiments, we used hyaluronic acid which should have bound water molecule as with mucins. For monitoring degradation of hyaluronic acid, we have developed affinity supported molecular matrix electrophoresis using 1%polyethyleneimine in polyvinyl alcohol. We have revealed that microwave-assisted heating of hyaluronic acid was dielectric heat in higher frequency than 1 to 2 GHz, while it was induction heat in lower frequency.

Free Research Field

複合糖質構造解析